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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/01/2020 |
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Data da última atualização: |
23/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. |
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Afiliação: |
VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINA CERQUEIRA SARMENTO OLIVARES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUANA RANGEL CÔRTES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. |
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DOI: |
10.22456/1679-9216.98317 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. MenosBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, ... Mostrar Tudo |
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Palavras-Chave: |
Billygoats; CTC. |
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Thesagro: |
Bode; Bovino; Caprino; Carneiro; Espermatozóide; Heparina; Ovino; Reprodução Animal; Sêmen. |
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Thesaurus Nal: |
Animal reproduction; Assisted reproductive technologies; Bulls; Cattle; Goats; Heparin; Rams; Sheep; Sperm capacitation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209450/1/cnpc-2019-Mini-Percoll.pdf
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Marc: |
LEADER 04821naa a2200469 a 4500
001 2119218
005 2020-01-23
008 2019 bl uuuu u00u1 u #d
024 7 $a10.22456/1679-9216.98317$2DOI
100 1 $aALFRADIQUE, V. A. P.
245 $aMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.$h[electronic resource]
260 $c2019
520 $aBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique.
650 $aAnimal reproduction
650 $aAssisted reproductive technologies
650 $aBulls
650 $aCattle
650 $aGoats
650 $aHeparin
650 $aRams
650 $aSheep
650 $aSperm capacitation
650 $aBode
650 $aBovino
650 $aCaprino
650 $aCarneiro
650 $aEspermatozóide
650 $aHeparina
650 $aOvino
650 $aReprodução Animal
650 $aSêmen
653 $aBillygoats
653 $aCTC
700 1 $aOLIVARES, C. C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aBALARO, M. F. A.
700 1 $aSARAIVA, H. F. R. de A.
700 1 $aCÔRTES, L. R.
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1707, p. 1-8, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 260 | |
| 101. |  | BARRETO, C. F.; MARTINS, C. R.; BRIXNER, G. F.; OLIVEIRA, D. B. de. Desempenho agronômico de cultivares de citros em semente implantado no município de Itaqui, Oeste do RS. In: ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 12., 2011, Fraiburgo. Levando conhecimento e tecnologia para a fruticultura: anais. Santa Catarina: EPAGRI, 2011. v. 2, resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 102. | | BARROS, I. de; RODRIGUES, G. S.; MARTINS, C. R. Desempenho ambiental da produção de coco verde: estudo de caso da fazenda Mumbuca em Conde (BA). In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS. 3., 2004, Brasília, DF; Aracaju, SE. Seifrut: [anais..]. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. p. 190-195.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 103. | | BARROS, I. de; RODRIGUES, G. S.; MARTINS, C. R. Desempenho Ambiental da Produção de Coco Verde: Estudo de caso da fazenda Mumbuca em Conde (BA). In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS. 3., 2004, Brasília, DF; Aracaju, SE. Seifrut: [anais..]. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. 197 p. Editores Técnicos: Carlos Roberto Martins, Embrapa Clima Temperado; Francisco Alisson Xavier, Embrapa Mandioca; Antônio Souza do Nascimento, Embrapa Mandioca e Fruticultura.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 108. | | CARVALHO, H. W. L. de; SOARES FILHO, W. dos S.; MARTINS, C. R.; PASSOS, O. S.; TEODORO, A. V.; CARVALHO, L. M. de; GIRARDI, E. A.; GESTEIRA, A. da S.; CARDOSO, B. T.; OLIVEIRA, T. R. A. de; MARQUES, M. G.; MOITINHO, A. C.; SANTOS, D. L. dos; PORTO, E. S.; ARAÚJO, S. B. de. Desempenho da laranjeira pineapple sobre diferentes porta-enxertos em áreas de Tabuleiros Costeiros de Sergipe. Aracaju, SE: Embrapa, 2015. (Embrapa Tabuleiros Costeiros. Comunicado Técnico, 174).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 109. | | CARVALHO, H. W. L. de; SOARES FILHO, W. dos S.; MARTINS, C. R.; PASSOS, O. S.; TEODORO, A. V.; CARVALHO, L. M. de; GIRARDI, E. A.; GESTEIRA, A. da S.; CARDOSO, B. T.; OLIVEIRA, T. R. A. de; MARQUES, M. G.; MOITINHO, A. C.; SANTOS, D. L. dos; PORTO, E. S.; ARAÚJO, S. B. de. Desempenho da laranjeira valência tuxpan sobre diferentes porta-enxertos em áreas de Tabuleiros Costeiros do Estado de Sergipe. Aracaju, SE: Embrapa, 2015. il. (Embrapa Tabuleiros Costeiros. Comunicado Técnico, 181).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 111. | | LEDO, A. da S.; SILVA JUNIOR, J. F. da; MARTINS, C. R.; AMORIM, E. P.; LEDO, C. A. da S. Desempenho de genótipos de bananeira nos tabuleiros costeiros, sergipe ? 2 ciclo de produção. In: REUNIÃO INTERNACIONAL ACORBAT, 20., 2013, Fortaleza. Acorbat: 40 anos compartilhando ciência e tecnologia. Fortaleza: Instituto Frutal: Acorbat Internacional, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Tabuleiros Costeiros. |
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| 112. | | SILVA, G. F. da; LIMA, A. D. V.; SOUZA, R. S. de; FARIAS, P. C. de M.; MARTINS, C. R. Desenvolvimento inicial de porta-enxertos de nogueira-pecã a campo e ambiente protegido em sistema orgânico. In: SIMPÓSIO SUL-AMERICANO DA NOZ-PECÃ, 2., 2019, Cachoeira do Sul. Anais. Brasília, DF: Embrapa, 2019. 119 p. Carlos Roberto Martins, Editor técnico (CPACT). p. 35-37.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 113. | | MARTINS, C. R.; BARROS, I. de; RODRIGUES, G. S.; SOUZA, M. E. de. Desenvolvimento metodológico para análise econômica ambiental de sistemas consorciados a base de citros nos Tabuleiros Costeiros. In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3.; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS, 3., 2014, Brasília, DF; Aracaju, SE. Anais... Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. p. 77-81.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 114. | | MARTINS, C. R.; BARROS, I. de; RODRIGUES, G. S.; SOUZA, M. E. de. Desenvolvimento Metodológico para Análise Econômica Ambiental de Sistemas Consorciados a Base de Citros nos Tabuleiros Costeiros. In: SEMINÁRIO SOBRE INTENSIFICAÇÃO ECOLÓGICA DA FRUTICULTURA, 3.; REUNIÃO COMITÊ GESTOR DO PROJETO SISTEMA ECOLOGICAMENTE INTENSIVO DE PRODUÇÃO DE FRUTAS, 3., 2014, Brasília, DF; Aracaju, SE. Seifrut: [anais..]. Aracaju, SE: Embrapa Tabuleiros Costeiros, 2014. p. 77-81. Editores Técnicos: Carlos Roberto Martins, Embrapa Clima Temperado; Francisco Alisson Xavier, Embrapa Mandioca; Antônio Souza do Nascimento, Embrapa Mandioca e Fruticultura.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 117. | | SOUZA, R. S. de; SILVA, G. F. da; LIMA, A. D. V.; HELLWING, C. G.; MARTINS, C. R.; MELLO-FARIAS, P. Diferentes concentrações de húmus líquido Em mudas de nogueira-pecã. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 28.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 21.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 5., 2019, Pelotas. Anais... Pelotas: UFPel, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 118. | | SILVA, M. S.; MARTINS, C. R.; BEZERRA, I.; INOUE-NAGATA, A. K.; AVILA, A. C.; RESENDE, R. O. Diversidade da proteina de movimento NSm de tospovirus e seu possivel envolvimento na interacao virus-hospedeiro. Fitopatologia Brasileira, Brasilia, v.23, p.325, ago. 1998. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 120. | | NACHTIGAL, J. C.; MARTINS, C. R.; SCHWENGBER, J. E.; ANTUNES, L. E. C.; RASEIRA, M. do C. B.; FRANZON, R. C.; BONOW, S. Diversificação produtiva em fruticultura orgânica. In: WOLFF, L. F.; EICHOLZ, E. D. (ed.). Alternativas para a diversificação da agricultura familiar de base ecológica - 2021. Pelotas: Embrapa Clima Temperado, 2021. 46 p. (Embrapa Clima Temperado. Documentos, 512). p. 13-15.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 260 | |
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